Sunday, October 27th, 2024

9:00 to 12:00 PM     Sunday Morning Workshop – Poly-/Oligonucleotide separation in biopharmaceutical processing and their quality requirements.  Coral Reef Instructor: Dr. Egbert Müller

2:00 to 5:00 PM       Sunday Afternoon Workshop Purification and characterization of protein superstructures and gene therapy vehicles. Coral Reef Instructor: Alois Jungbauer

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6:00 to 7:00 PM        Welcome Reception on Ocean Terrace (Hotel Patio) Open to All Registrants and Accompanying Persons – Sponsored by Solventum

Monday, October 28th, 2024

7:30 AM Symposium Registration Open

10:00 AM Exhibition Opens

7:30 – 8:30 Continental Breakfast

8:30 AM Opening Remarks – Dr. Barry Boyes, Symposium Co-Chair

Oral Session 1: Advancing Approaches for Biomolecule Analysis –

Session Chair: Dr. Brian Wei

8:35 AM Nanoflow Need Not Apply- Analytical Flow HRIM-MS Works to Resolve Native Protein-Ligand Binding, Ashli Simone, MOBILionSystems, Chadds Ford, PA USA

9:00 AM Unveiling Column Interchangeability in UHPLC through Multivariate Modeling: A Case Study, Imre Molnár, Molnar-institute for Applied Chromatography, Berlin, Germany

9:25 AM How Complementary are HILIC, Ion Mobility, and MS for The Analysis of N-glycan Isomers?, Hoang Kim Ngan Thai, University of Georgia, Athens, GA, USA

9:50 AM Targeted Metabolite Analysis Using LC-MS/MS with Parallel Reaction Monitoring (PRM) Oluwatosin Daramola, Dept. Biochemistry and Chemistry, Texas Tech University, Lubbock, TX, USA

10:15 AM Break, Exhibits, and Poster session (posters up throughout the conference exhibition)

Oral Session 2: Bionanoparticles –

Session Chair: Prof. Alois Jungbauer

10:45 AM Purification of Bionanoparticles, Prof. Alois Jungbauer, Institute of Bioprocess Science and Engineering, BOKU University, Vienna, Austria

11:10 AM Characterization of drug delivery vectors using Field-Flow Fractionation coupled with Light Scattering, Dr. Soheyl Tadjiki, Postnova Analytics GmbH, Landsberg, Germany

11:35 AM Novel Technologies for Large Particle Separations and Control in Bioprocessing, Dr. Alexei Voloshin, Global Head Bioprocess Science, Solventum, St Paul, MN, USA

Lunch Break, on-own, or vendor seminar

12:10–1:30 PM Free Vendor Seminar Sponsored By Agilent  – Complementary Methodologies for Size Heterogeneity Analysis of Antibody Impurities (Box Lunch Provided. Must register online or at Agilent Booth) – Salon E, lecture hall.

12:10–1:30 PM Free Vendor Seminar Sponsored By Waters & Wyatt tech – Advanced LC Technologies for Increasingly Complex Molecule (Box Lunch Provided. Must register online or at Waters Booth) – Salon D

1:30 – 2:15 PM Poster Session in the Exhibition Hall

Oral Session 3: Newer Therapeutic Modalities –

Session Chair: James Martosella

2:15 PM Critical Method and Procedural Improvements for Optimizing an siRNA Oligonucleotide Ion Pairing Reversed-Phase Liquid Chromatography (IP-RPLC) Release Assay, James Martosella, GlaxoSmithKline, Philadelphia, PA, USA

2:40 PM Characterization of guide RNA for CRISPR: Methods, Mechanisms and Applications, Bingchuan Wei, Synthetic Molecule Chemistry, Genentech, South San Francisco, CA, USA

3:05 PM An LC-based, Multi-attribute Method Supporting the Process Development for Non-viral Gene Therapy Wei-Chiang Chen, BioProcess Analytics, Genomic Medicine Unit, Sanofi, Boston, MA, USA

3:30 PM Coffee Break in Exhibit Hall

Oral Session 4: Process Technology and Analytics –  

Session Chair: Dr. Todd Maloney

4:00 PM PLENARY SPEAKER: Process Analytical Technology for Advanced Process Understanding and Control: Case Studies in Synthetic Drug Substance Process Development and Manufacturing, Todd Maloney, Vice President of Synthetic Molecule Design and Development, Eli Lilly, Indianapolis, IN, USA

5:00 PM A comprehensive approach to the quantification and characterization of double-stranded RNA (dsRNA) products as a new generation of crop protection agents), Nicholas Skizim, Analytical Sciences, Greenlight Biosciences, Medford, MA, USA

5:25 PM “Is it any good?” The science-driven evaluation system of bulk silica phases for large-scale peptide API purification, Imre Sallay, Osaka Soda Co. Osaka, Japan.

5:50 PM Keratin recovery and application: process development and techno-economic insights, Cariny Polesca, Department of Chemistry, University of Aveiro, Aveiro, Portugal

6:15 – 7:00 PM Social Mixer & Poster Session in Exhibit Hall – Sponsored by Shimadzu

Tuesday, October 29, 2024

7:30 Symposium Registration Open

7:30–9:00 AM Free Vendor Seminar Sponsored By Tosoh – TBD  (Breakfast Provided. Must  Pre-register at Tosoh Booth) – Salon E, lecture hall.

10:00 AM Exhibition Open

Oral Session 5: Quality by Design – Process, Purification and Analytical Session Chair: Dr. Emily Schirmer

8:45 AM Analytical Quality by Design and its Role in Accelerating the Process Development and Biomanufacturing of Monoclonal Antibodies, Matthew Dalphin, Catalent Pharma Solutions, Madison, WI, USA

9:10 AM Step Gradient Simulated Moving Bed Purification Process for Anion Exchange Chromatography, Jonas Wege, Tosoh Bioscience GmbH Weiterstadt, Hessen, Germany

9:35 AM Development and Optimization of SFC Polypeptide Separations Using Automation and QbD Principles, Brian Domanski, Shimadzu Scientific Instruments, Inc., Columbia, MD, USA

10:00 AM Coffee Break – Sponsored by Solventum

Oral Session 6: Higher Order Structure Analysis –      

Session Chair: Prof. Ronald Orlando

10:20 AM PLENARY SPEAKER: Protein Footprinting and Applications for Soluble and Membrane Proteins, Michael L. Gross, Chemistry, Immunology and Internal Medicine (School of Medicine), Washington University, St. Louis, MO, USA

11:20 AM Unveiling Structural-Function Relationship through Hydrogen Deuterium Exchange Coupled with Mass Spectrometry (HDX-MS), Bingchuan Wei, Synthetic Molecule Chemistry, Genentech, South San Francisco, CA, USA

11:45 AM Reduced back-exchange and carryover with subzero temperature chromatography system for HDX-MS, Kyle Anderson, Bioprocess Measurements Group, Biomolecular Measurement Division National Institute of Standards and Technology, Rockville, MD, USA

Lunch Break, on-own, or vendor seminar

12–1:30 PM Free Vendor Seminar Sponsored By Thermo Fisher Scientific – Effective and simultaneous aggregate and HCP reduction via flow-through mixed-mode chromatography in Mab purification- Detailed HCP clearance analysis by UHPLC-HRAM MS/MS. (Box Lunch Provided. Must  Pre-register at Thermo Booth)

12:10–1:30 PM Free Vendor Seminar, Sponsored By i3 – Digital Membrane Chromatography (Box Lunch Provided. Must register online or at i3 Booth)

Oral Session 7: Nucleic Acid Separations –

Session Chair: Dr. Bingchuan Wei

1:30 PM Characterization of Oligonucleotides (siRNA) and Related Impurities Using LC/MS and Potential Analytical/Biophysical Technologies Eric Shi, GSK – Boston, MA, USA

1:55 PM Pre-purification of minicircle DNA from cell lysates with ionic liquid-based aqueous biphasic systems, Ana I. Valente, Aveiro Institute of Materials CICECO – Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal

2:20 PM Examining Pore Size Effects for Superficially Porous Particles Applied to Ion Pairing-Reversed Phase Separations of Nucleic Acids, Barry Boyes, Advanced Materials Technologies, Inc., Wilmington, DE USA

2:45 PM Making Orthogonal Analytics Work for You: A Comprehensive and Targeted Approach to mRNA Characterization, Brian Roper, PTC, Genentech, South San Francisco, CA, USA

2:30 PM Snack Break in exhibit hall

Oral Session 8: Glycomics/glycoproteomics –

Session Chair: Prof. Yehia Mechref

3:40 PM Advanced Meso-Graphitic Carbon Materials for Enhanced LC-MS/MS Glycomics and Glycoproteomics Analysis, Yehia Mechref, Dept. Biochemistry and Chemistry, TTU Center for Biotechnology and Genomics, Texas Tech University, Lubbock, TX, USA

4:05 PM Comprehensive Targeted Analysis of Glycans and Glycopeptides by LC-MS/MS with Parallel Reaction Monitoring (PRM), Cristian Gutierrez Reyes, Dept. Biochemistry and Chemistry, TTU Center for Biotechnology and Genomics, Texas Tech University, Lubbock, TX, USA

4:30 PM  TBA, Jonathan Amster, Department of Chemistry, University of Georgia, Athens, GA, USA

4:55 PM A multi-glycomic workflow for characterizing the glycocalyx in the cell membrane, Carlito Lebrilla, University of California, Davis, CA, USA

6:00 PM Dinner and Drinks in the Sea Breeze ballroom (tickets available for purchase for all attendees and accompanying persons)

Wednesday, October 30th, 2024

7:45 AM Symposium Registration Open – Continental Breakfast

Oral Session 9: Fundamentals of Separations –

Session  Chair: Dr. Mark Schure

8:30 AM Molecular Simulations and Retention Measurements of Hydrophilic Interaction Liquid Chromatography (HILIC): How HILIC works, Mark Schure, Kroungold Analytical, Blue Bell, PA USA

9:10 AM Peptide separation selectivity of reversed-phase C18 separation media for proteomic applications, Oleg Krokhin, Internal Medicine, University of Manitoba, Winnipeg, MB Canada

9:35 AM Exploring the potential of polymeric ionic liquids as chromatographic supports for Interferon downstream processing, Leonor Castro, Aveiro Institute of Materials CICECO – Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal

Oral Session 10: Protein Therapeutic Separations –

Session Chair: Dr. Barry Boyes

9:50 AM Advancements in Determination and Characterization of DAR in Antibody Drug Conjugates, Li Cao, Strategic External Development, GlaxoSmithKline, Philadelphia, PA, USA

10:15 AM Native HIC MS of ADCs for DAR species determination and identification, Lance Cadang, Genentech, South San Francisco, CA, USA

10:40 AM Breaking barriers: Avian Immunoglobulin Y (IgY) antibodies to fight Antimicrobial Resistance, Catarina Almeida, CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal

11:05 AM Coffee Break

11:25 AM High-Throughput Analytical Assay Development for N-Glycans in Novel Protein-Based Modalities, Brian Wei, Bioprocess Analytics, Sanofi, Boston, MA, USA

11:50 AM Identifying the Absolute Stereochemical Configuration of Biopolymer Building Blocks with Ion Mobility-Mass Spectrometry, Mya Brown, CCRC, University of Georgia, Athen, GA USA

12:15 PM Defining the Role of Glycosylation in Traumatic Brain Injury: An Innovative Analysis of IgG Glycosylation, Sherifdeen Onigbinde, Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX USA

12:40 PM Detection of cancer biomarkers using microfluidic devices  assisted by serum pretreatment with ionic liquid-based aqueous biphasic systems, Maria Mendes, Department of Chemistry, University of Aveiro, Aveiro, Portugal

1:05 PM  Closing Remarks and Invitation to 43rd ISPPP:

Dr. Barry Boyes and Prof. Alois Jungbaue