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Monday October 19 Breakfast Seminars
Title Coming Soon
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Mastering the art of oligonucleotide analysis via LCMS using the HALO 1000Å OLIGO C18 HPLC column
Presented By: Josh McBee Ph.D.
Oligonucleotide-based therapeutics are a rapidly growing area that present unique challenges for analytical analysis. LC/MS has excellent utility for performing critical quality attribute (CQA) testing but can present several novel hurdles for MS users accustomed to performing routine small molecule, protein, or peptide analysis. Their large size, hydrophilic nature and separation challenges require unique approaches.
This seminar will demonstrate how to build an ion pair/reversed phase (IP/RP) LC/MS workflow using our novel HALO 1000Å OLIGO C18 HPLC columns. We will discuss proper sample prep and handling, ion pairing reagent selection and procedures for making IP buffers, and how to minimize adduction risk when performing MS analysis.
We will show several applications including siRNA impurity analysis and sgRNA’s for CRISPR applications.
We hope this seminar will provide MS users with confidence in approaching oligonucleotide analysis and a framework for proper method development.
Monday October 19 Lunch Seminars
Vaast: An Innovative Solution for Amino Acid Analysis
Presented By: Dr. Weston J. Umstead
Amino acid analyses have historically provided chromatographers with many challenges, stemming from the diversity in functionality, and the complex matrices they’re often isolated from. Furthermore, their chiral separations have often been overlooked because of a lack of a unified tool set to perform such analyses quickly. While there are some solutions available, they typically require 2D chromatography setups, multiple columns and mobile phases, or messy and unreliable derivatization steps.
That has changed now with the introduction of Vaast, Daicel’s first-of-its-kind analytical solution to separate 21 proteinogenic amino acids in a single analytical method. This includes the achiral pairs like D from D and L from L, but also the chiral D and L pairs from each other. Beyond these 21 proteinogenic amino acids, Vaast is also able to resolve protected amino acids, non-canonical amino acids, isobaric amino acid isomers, and other amino acid-containing compounds.
This seminar will focus on the history of these separations, including relevant background and previous solutions for these separations, and help contextualize the impact of this next generation solution, Vaast, for enhancing and improving amino acid separations.
Title Coming Soon
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Tuesday October 20 Breakfast Seminars
Title Coming Soon
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Integrated and intensified downstream processing within a continuous multi-column chromatography platform
Presented By: Abbie Breton
Achieving true continuity in downstream processing remains a key challenge for monoclonal antibody (mAb) production. Here, multi-column chromatography (MCC) is advanced beyond continuous capture to enable direct integration of a subsequent chromatographic purification step on a single system.
Previous integration of downstream steps has been constrained by equipment and process limitations. The adapted multi-column system used in this work integrates two chromatographic steps in a single 8-column setup, supporting uninterrupted operation and efficient resin use.
Using a high alkaline stable, fast kinetic ligand, and high dynamic binding capacity (DBC) Protein A affinity resin, we demonstrate robust mAb capture and subsequent polishing with a highly salt-tolerant cation exchange resin, advancing practical implementation of end-to-end continuous purification. In addition, a low-threshold pH step was applied into this process integration to achieve inline virus inactivation. A high degree of automation has been achieved with this setup, while the footprint has been reduced both by reducing the size of the systems and by saving on cost of buffers, thereby making downstream processing more sustainable.
Tuesday October 20 Lunch Seminars
Title Coming Soon
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Bulk silica for large-scale peptide purification by HPLC
Presented By: Dr. Imre Sallay, Mr. Jonathan Edelman
The DAISOGEL Team will present our newest products and our approaches to solving your peptide(s) purification needs. We will highlight the science behind the new stationary phases and highlight their usefulness for peptide purification. Our manufacturing capacity for RP-HPLC silica is unmatched globally, as is our service.
