ISPPP

Sunday Workshop Information

Please make your selection at our registration site.

Workshop Fees (Per Session):          Industry/Academia/Government: $100          Students:$50

Sunday Afternoon Workshop #1     12:30 PM – 2:30 PM     (Main Hall)

 

Separation of bionanoparticles: biophysical principles and new materials
Instructor: Dr. Alois Jungbauer
Department of Biotechnology, BOKU University, Vienna, Austria,  Austrian Centre of industrial Biotechnology, Vienna Austria
Abstract:
State of the art technologies for the separation of viruses, virus-like particles (VLPs), and extracellular vesicles—collectively referred to as bionanoparticles—typically combine at least one chromatographic step with multiple membrane-based separation processes. Conventional chromatography media, originally developed for protein purification, are inherently limited in this context due to restricted mass transfer and/or low binding capacity. In order to understand the separation challenges the biophysical properties of bionanoparticles will be reviewed. To address the limitations of traditional media, advanced formats such as membrane adsorbers, monoliths, and nonwoven materials have been introduced, offering enhanced binding capacity and reduced mass transfer constraints. In this workshop examples will be presented including the purification of VLPs from cell culture supernatants, the separation of VLPs from extracellular vesicles, and the processing of large viruses such as the measles virus. Furthermore, perspectives on the design of ideal separation strategies for bionanoparticles, including key properties required for next-generation chromatographic materials will be outlined. The potential impact of rapid virus detection methods on process monitoring, control, and development will be also discussed.

Sunday Afternoon Workshop #2     3:00 PM – 5:00 PM     (Main Hall)

 

Mass Spectrometry-Based Glycomics and Glycoproteomics: From Fundamentals to Advanced Isomer-Resolved Workflows
Instructor: Dr. Yehia Mechref
Robert A. Welch Endowed Chair in Chemistry, Paul W. Horn Distinguished Professor, Founding Director of the Human Molecular Aging Center, and Director of the Center for Biotechnology and Genomics at Texas Tech University.
Abstract:
Glycomics and glycoproteomics represent rapidly expanding frontiers in analytical chemistry, driven by the critical roles of glycans and glycoproteins in biological regulation, disease progression, and therapeutic development. However, their comprehensive analysis remains challenging due to structural complexity, microheterogeneity, and extensive isomeric diversity.
This workshop will provide a comprehensive, hands-on-oriented overview of mass spectrometry (MS)-based strategies for glycan and glycoprotein analysis, spanning fundamental principles to state-of-the-art methodologies. Participants will gain practical insight into sample preparation workflows for released glycans and intact glycopeptides, including enzymatic and chemical approaches, derivatization strategies, and enrichment techniques.
A central focus will be placed on liquid chromatography–mass spectrometry (LC–MS) platforms for high-resolution separation and structural characterization. Advanced chromatographic techniques, including porous and mesoporous graphitized carbon and hydrophilic interaction chromatography, will be discussed in the context of resolving glycan and glycopeptide isomers. Complementary MS/MS fragmentation methods (HCD, ETD, EThcD, and emerging approaches) will be presented for confident structural elucidation.
The workshop will also cover quantitative strategies, data acquisition modes, and bioinformatics tools for high-throughput glycomics and glycoproteomics, with emphasis on improving confidence, reproducibility, and scalability. Real-world applications will be highlighted through case studies in biomarker discovery, including analyses of serum and extracellular vesicles in cancer, neurodegenerative diseases, and other clinical contexts.
By integrating theory with practical guidance and representative datasets, this workshop aims to equip participants with the knowledge and tools needed to design, implement, and interpret advanced MS-based glycomics and glycoproteomics experiments, enabling them to address complex biological and translational research questions.